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Title

MONITORING OF LUMINESCENT BACTERIA IN SHRIMP FARM OF PERSIAN GULF

Writers

MOOSAVI K.

Pages

 Start Page | End Page

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Abstract

 BACKGROUND AND PURPOSE:MARINE BACTERIAL LUMINESCENCES ARE THE MOST INTERESTED BACTERIA BECAUSE OF THEIR IMPORTANCE IN ECOLOGICAL AND BIOTECHNOLOGICAL ASPECTS. THERE ARE POOR INFORMATION OF PRESENCE OR ABSENCE OF THESE MICROORGANISMS IN IRANIAN NATURAL AND ARTIFICIAL ENVIRONMENTS. IN THIS STUDY WE HAVE TRIED TO VERIFY THE PRESENCE OR ABSENCE OF THESE BACTERIA IN THE NATURAL POND WATER THAT ARE RELATED TO PERSIAN GULF SHRIMP.MATERIALS AND METHODS:SAMPLES WERE TAKEN FROM TWO FARMS (9 POOLS) IN BUSHEHR SHRIMP. THE WATER WERE CULTURED IN THE TCBS MEDIUM (VIBRIO-SPECIFIC CULTURE MEDIUM) CONTAINING 1.5% SALT (PH=7.2) AND INCUBATD AT 25 AND 37OC. THE OBSERVED COLONIES WERE PURIFIED BY STREAKING AND TRANSFERRED TO SWC. THE BIOLUMINESCENCE BACTERIA WERE QUALIFIED BY BIO-LUMINOMETRY. THE THREE COLONIES WITH THE HIGHEST INTENSITY WERE SELECTED FOR THE MOLECULAR APPROACHES. SOME OF THE CLASSICAL BIOCHEMICAL TESTS, SUCH AS GELATINASE, DNASE, INDOLE AND LYSINE DECARBOXYLASE WERE USED FOR BACTERIAL IDENTIFICATION.RESULTS:MICROSCOPIC OBSERVATIONS SHOWED THAT ALL OF ISOLATES ARE BELONG TO GRAM-NEGATIVE BACTERIA. THE GROWTH TEMPERATURE FOR ISOLATES OF LUMINESCENCE 1B, 2B WAS 25OC AND 37OC FOR 1A. LIGHT INTENSITY WAS 30000000 RUL/S FOR THREE ISOLATES. INDOLE PRODUCTION, DNASE, GLUCOSE FERMENTATION, LYSINE DECARBOXYLATION, H2S PRODUCTION AND MOBILITY FOR THESE BACTERIA WERE POSITIVE. HOWEVER GELATINASE ACTIVITY WAS POSITIVE FOR 1A AND 1B, WHILE POSITIVE FOR 2B NEGATIVE.DISCUSSION:ACCORDING TO THE MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION, THE ISOLATES ARE SIMILAR TO VIBRIONACEA FAMILY, GENUS VIBRIO.CONCLUSION:THE APPLIED METHODS OF PRESENT STUDY HAVE BEEN USEFUL TO TRACK AND ISOLATE THE LUMINESCENCE BACTERIA AND CAN BE A GOOD MODEL FOR INVESTIGATING OF THE OTHER MICROORGANISM IN NATURAL ENVIRONMENTS. THE OTHER MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS MUST BE DONE AS WELL AS OF THE LUMINESCENCE INTENSITY TO DETERMINE THE DIFFERENCE BETWEEN THE GENUS AND SPECIES TO PREPARE OF THE OTHER STUDIES, ESPECIALLY MOLECULAR STUDIES.

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