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Title

USE OF PCR-RFLP FOR ASSESSMENT OF GENETIC DIVERSITY AMONG XANTHOMONAS SPP.

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Abstract

 THE GENUS XANTHOMONAS IS FORMED BY A COMPLEX GROUP OF BACTERIAL SPECIES WITH DIVERSE PHYSIOLOGICAL AND PHYTOPATHOLOGICAL TRAITS. THIS GROUP OF PHYTOPATHOGENS INFECTS MANY ECONOMICALLY IMPORTANT PLANTS SUCH AS COTTON, BEANS, RICE, CRUCIFERS AND THEREFORE, THESE BACTERIA REPRESENT A THREAT TO AGRICULTURE AROUND THE WORLD. SOME OF THE MOST IMPORTANT DISEASES CAUSED BY XANTHOMONAS SPECIES INCLUDE CITRUS CANKER AND BLACK ROT, ADDITIONALLY XANTHOMONAS IS ALSO IMPORTANT IN BIOTECHNOLOGY DUE TO THE PRODUCTION OF A POLYSACCHARIDE KNOWN AS A XANTHAN GUM, WHICH IS USED AS A STABILIZER AND EMULSIFIER IN THE FOOD AND COSMETIC INDUSTRY, AMONG OTHER APPLICATIONS. IN THIS STUDY GENETIC DIVERSITY AMONG 15 STRAINS OF XANTHOMONAS SPP. WERE CLARIFIED BY PCR RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) ANALYSIS OF 16S-23S RDNA INTERNAL TRANSCRIBED SPACER (ITS) REGION USING FOUR RESTRICTION ENZYMES INCLUDING ALUI, MBOI, DDEI, BSNI. TOTAL BACTERIAL DNA WAS PREPARED BY USING PHENOL- CHLOROFORM METHOD. PCR WAS PERFORMED BY USING ITS PRIMER, PCR PRODUCTS WERE DIGESTED WITH RESTRICTION ENZYMES. THREE ENZYMES INCLUDING ALUI, DDEI, BSNI SHOWED TWO DIFFERENT PROFILES AMONG ALL STRAINS BUT ONE ENZYME, MBOI, SHOWED THREE DIFFERENT PROFILES. PCR- RFLP ANALYSIS OF IT’S IS A SIMPLE AND RAPID TECHNIQUE TO EVALUATE BIODIVERSITY AMONG XANTHOMONAS SPP. AND CAN USE FOR PARTIALLY DIFFERENTIATION AMONG XANTHOMONAS SPECIES.

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