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Title

THE DRAFT GENOME SEQUENCE OF NESERENKONIA SP STRAIN F ISOLATED FROM ARAN-BIDGOL SALT LAKE IN IRAN

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Abstract

 THE DRAFT GENOME OF THE AEROBIC, GRAM-POSITIVE, CHEMOORGANOTROPH AND HALOPHILIC NESTERENKONIA SP STRAIN F CONSISTS OF 2,812,133 BP CHROMOSOME (AFRW01000000 ACCESSION NO.). THE GENOME PROJECT DATA IS ALSO AVAILABLE AT GENBANK UNDER THE GENOME PROJECT ID 68173.NESTERENKONIA IS AN AEROBIC, GRAM-POSITIVE, CHEMOORGANOTROPH, CATALASE POSITIVE, MESOPHILIC AND HALOPHILIC GENUS FROM MICROCOCCACEAE FAMILY. NESTERENKONIA SP STRAIN F WAS ISOLATED FROM ARAN-BIDGOL HYPERSALINE LAKE IN IRAN AND CHARACTERIZED TO PRODUCE THE HALOPHILIC AND ORGANIC SOLVENT TOLERANT Α-AMYLASES. DESPITE OF ITS HIGH POTENTIAL FOR PRODUCING THE VARIOUS NATURAL COMPOUNDS AND SPECIALIZED MECHANISMS FOR ADAPTION TO EXTREME CONDITIONS, THERE IS A LITTLE INFORMATION ABOUT THE GENETIC AND GENOME FEATURES OF THE NESTERENKONIA GENUS.GENOME SEQUENCING OF NESTERENKONIA SP STRAIN F WAS PERFORMED USING 454 GS-FLX TITANIUM. WE OBTAINED A TOTAL 201,320 RANDOM READS, COVERING A TOTAL OF 44,347,672 BP. THE APPROXIMATE COVERAGE OF NESTERENKONIA SP STRAIN F GENOME WAS 16 FOLD. THE SEQUENCE READS WERE ASSEMBLED INTO 138 CONTIGS USING NEWBLER ASSEMBLER SOFTWARE V. 2.3. THEN, THE RAST ANNOTATION PIPELINE WAS USED TO ANNOTATE THE DNA SEQUENCE.THE G+C CONTENT OF THE DRAFT GENOME WAS 71.5% THAT WAS SIMILAR TO PREVIOUS REPORT BY STACKEBRANDT ET. AL. THE SUM OF 138 LARGE CONTIGS SIZE OF NESTERENKONIA SP STRAIN F IS 2,812,133 BP. THE DRAFT GENOME CONTAINED 2484 GENES WITH 1794 NON-HYPOTHETICAL AND 690 HYPOTHETICAL PROTEIN CODING SEQUENCES AND 50 STRUCTURAL RNAS.ANALYSIS OF ANNOTATED GENOME SEQUENCE OF NESTERENKONIA SP STRAIN F REVEALED THE PRESENCE OF GENES INVOLVED IN PRODUCTION OF Α-AMYLASES INCLUDING MALTODEXTRIN GLUCOSIDASE AND ALPHA-GLUCOSIDASE.NESTERENKONIA SP STRAIN F COMPRISES CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS (CRISPR), WHICH GRANTS AN EFFECTIVE MECHANISM AGAINST FOREIGN GENETIC ELEMENTS AS AN ADAPTIVE IMMUNE SYSTEM (4). THE CRISPR-ASSOCIATED HELICASE CAS3, RECB FAMILY EXONUCLEASE CAS4B AND CRISPR-ASSOCIATED PROTEIN CAS1 HAVE BEEN DETECTED USING CRISPRFINDER ONLINE PROGRAM.

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