Seminar Paper

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Title

DIVERSITY OF SECRETORY CARBOHYDRASES AMONG YEASTS ISOLATED FROM ENVIRONMENTAL SAMPLES

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Abstract

 YEASTS ARE, ISOLATED FROM FOOD RESOURCES, KNOWN AS LEADING PRODUCERS OF FERMENTATION PRODUCTS OF OENOLOGICAL IMPORTANCE AND SINGLE CELL PROTEIN. ALTHOUGH YEASTS FROM ENVIRONMENTAL RESOURCES HAVE LESS BEEN STUDIED THAN THOSE OF FOOD ORIGINS, BUT THE ENVIRONMENTAL ISOLATES DEMAND MORE ATTENTION BECAUSE OF THEIR WIDE POTENTIAL APPLICATIONS. IN THIS STUDY, SAMPLES WERE COLLECTED FROM DIFFERENT MANMADE AND NATURAL ENVIRONMENTAL RESOURCES AND CULTIVATED ON APPROPRIATE CULTURE MEDIA. OUT OF 107 NUMBERS OF ISOLATES ONLY 38 NUMBERS WERE ABLE TO PRODUCE ONE OF TESTED CARBOHYDRASES. 35.1% OF THE YEAST ISOLATES WERE ABLE TO DEGRADE AT LEAST ONE OF THE POLYSACCHARIDES TESTED. 4% OF THE YEAST ISOLATES WERE ABLE TO DEGRADE TWO OR MORE OF POLYSACCHARIDES. PRODUCTION OF SECRETORY ENZYMES INCLUDING XYLANASE, AMYLASE AND CELLULASE WAS SHOWN. NONE OF ISOLATES WERE ABLE TO DEGRADE CHITIN. THE ONLY ISOLATE WITH THE ABILITY TO DEGRADE ALL THREE POLYSACCHARIDES WAS BIOCHEMICALLY AND MOLECULARLY CHARACTERIZED BY SEQUENCING OF D1/D2 REGION OF 26S RDNA AND TENTATIVELY IDENTIFIED AS A CRYPTOCOCCUS SP.

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