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Information Seminar Paper

Title

POLYCLONAL ANTIBODY PRODUCTION AGAINST ARTEMISININ EXTRACTED FROM ARTEMISIA SIEBERI

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Abstract

 Artemisinin (Art) has a long time therapeutic history and extensive application in traditional and modern medicine such as anti malarial effect. Although the anticoccidial effect of Art has been studied somewhat, there is no report about its pharmacodynamics in coccidiosis. The aim of this study was to produce polyclonal antibody (Ab) against Art, labeling and using it to trace the site of action of Art in coccidiosis by using immunohistochemsitry method. Since Art is a hapten, it was necessary to conjugate it with a suitable carrier to be immunogenic; therefore a carboxyl group was added to Art by using mixed anhydride method. Then this reactive compound of Art was conjugated with BSA. Immunization with 0.6ML of Art+BSA conjugate was done (IM) in rabbit in two times. In the first injection, the Freund complete adjuvant and in the 2nd injection Freund incomplete adjuvant was used. After two weeks, blood samples were collected, and their serums were separated. Immunoblots technique was used to assay the production of Ab. Saturated ammonium sulphate was used to separation and concentration of Ab and FITC for labeling it. This assay showed that the Ab has been successfully produced against artemisinin in rabbits. This Ab is a fundamental step for pharmacodynamic studies and to detect artemisinin disposition in the blood and body tissues.

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