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Information Seminar Paper

Title

CHARACTERIZATION OF K CHANNEL GATING ON ROUGH ENDOPLASMIC RETICULUM OF RAT HEPATOCYTE

Writers

SEPEHRI H. | ELIASI A.

Pages

 Start Page | End Page

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Abstract

 Introduction: endoplasmic reticulum is a universal signaling organelle which regulates a wide range of hepatocyte functions. Therefore identification and characterization of ER ion channels is important. Existence of K+ channel in the hepatocyte endoplasmic reticulum (ER) has been recently reported. In this study we examined channel gating properties in various voltages.Methods: Hepatocyte ER vesicles were isolated and incorporated in to the bilayer lipid membrane (BLM). The BLM was formed by phosphatidylcholine on the 350 µm aperture between two chambers (cis chamber containing 200mM KCl and Trans chamber containing 50mM KCl). Single channel recording and dwell time analysis were used for channel gating characterization.Results: dwell time analysis revealed that the channel have two closed and two open state at 0 mV and a new additional long lasting open state was observed in 40mV.Conclusion: This observation suggests that new conformationarmationalone new log lasting open state was adeddepolarization can produce one new conformation in the channel which can induce long lasting opening.

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