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Information Seminar Paper

Title

MMUNO-BIOCHEMICAL COMPARISON OF NITRIC OXIDE AND REACTIVE NITROGEN INTERMEDIATES (RNI) PRODUCTION IN SUSCEPTIBLE BALB/C AND RESISTANT C57BL MICE INFECTED WITH LEISHMANIA MAJOR

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Abstract

 Background: Cutaneous infection caused by protozoa the genus Leishmania is a major worldwide health problem, with high endemicity in developing countries including Middle East, Africa and Latin America. NO represents a key mechanism in the elimination of parasite from the body. In the current study, we compared production of nitric oxide and reactive nitrogen intermediates (RNI) in two different hosts (susceptible BALB/c and resistant C57BL mice) infected with L. major.Methods: To carry out this investigation, mice were assigned to 4 groups (each with 5 mice) as BALB/c infected with L. major, control BALB/c, C57BL infected with L. major and control C57BL. Exprimental Leishmaniasis was initiated by subcutaneous (s. c.) injection of the 2×106 promastigotes into the basal tail of two groups. The development of lesions was determined weekly by measuring the two diameters. After 10 weeks, all mice were killed humanly and target tissues including lymph node, spleen and liver from each mouse were removed, weighted and their impression smears were also prepared. Disease period, macroscopic features, lesion size, RNI level in plasma and in liver and spleen suspensions, proliferation of amastigotes inside macrophages, visceralization of parasite and hepatosplenomegaly in both susceptible and resistant mice infected with L. major was compared.                            Results: Results from this investigation clear that differences between susceptible BALB/c and resistant C57BL mice were correlated with immuno-biochemical factors and clearly point to a partial involvement of NO in the cytotoxic activity of macrophages against this parasite. The modulation of NO was able to modify clinical signs and could affect the proliferation of amastigotes inside macrophages, lesion sizes, survival rates, degree of splenomegaly/hepatomegaly and presence of amastigotes in lesion smears of liver, spleen and lymph node.

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