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Title

STUDY OF ROSE BENGAL-INDUCED CYTOTOXICITY AND APOPTOSIS IN BREAST CANCER CELL LINE (MCF-7)

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Abstract

 Introduction: Breast cancer is the most common type of cancer and the leading cause of cancer-related mortality in women. Current therapies for breast cancer are often limited by short-term efficacy due to the emergence of drug resistance In view of this, there is much interest in the identification of new agents for the treatment of breast cancer. Rose Bengal (RB) has been used as a photosensitiser in photodynamic treatment but in the present study, direct effects of RB, not as a photosensitiser, was tested in MCF-7 cell line in the absence of any light. Meanwhile role of reactive oxygen specious (ROS) were studied in RB-induced toxicity in MCF-7 cells.Methods: MCF-7 cells were cultured in DMEM medium. Cell viability was quantitated by MTT assay. Apoptotic cells were determined using PI staining of DNA fragmentation by flow cytometry (sub-G1 peak). ROS was measured using DCF-DA by flow cytometry analysis.Results: The result showed RB could induce pronounced cell death in MCF-7 cells as a concentration and time dependent manner. This toxicity was markedly induced by apoptosis. Although RB could increase ROS production in MCF-7 cells but pretreatment with antioxidant -glutamylcysteinylglycine (GSH) could not decrease RB-induced toxicity.Conclusion: In conclusion RB could be considered as a promising chemotherapeutic agent in breast cancer treatment in future.

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