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Information Seminar Paper

Title

CHONROPROTECTIVE EFFECTS OF POMEGRANATE FRUIT EXTRACT IN IODOACETATE-INDUCED OSTEOARTHRITIS ON TIBIOFEMORAL JOINT OF MICE

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Abstract

 Aims: To study the effectiveness of Pomegranate Fruit Extract (PFE) on osteoarthritis (OA), we used mono-iodoacetate (MIA), an inhibitor of glycolysis, in tibiofemoral joint of mice that promotes loss of articular cartilage similar to that noted in human OA. PFE exert anti-inflammatory effects, however, no histopathological studies have been undertaken in vivo to investigate whether it protect articular cartilage.Methods: We describe the histopathology in the subchondral bone and cartilage of mice knee joints treated with a single intra-articular injection of MIA (0.1 mg) and sacrificed14 and 28 days post injection. Then, the beneficial effect of PFE was studied in four groups, orally; Group 1: administration of PFE (4ml/kg), Group 2: administration of PFE (20ml/kg). Histopathological changes in knee joints were studied after two and four weeks.Results: Histologically, the early OA were characterized by areas of chondrocytes degeneration/necrosis sometimes involving the entire thickness of the articular cartilage in the tibial plateaus and femoral condyles. Changes to the subchondral bone and proteoglycane contents, were observed and also, there was focal fragmentation and collapse of bony trabeculae with fibrosis and necrosis. Synovial cell proliferation was noted. Interestingly, administration of PJ in different groups of mice prevented, the negative effects of iodoacetate, both dose and time dependently. Chondrocytes damages were significantly prevented and proteoglycane were less affected, especially in groups receiving high amount of PJ and no cell proliferation and inflammatory cell were detected in synovial.Conclusions: Fast and progressive damage to articular cartilage is induced by single intra-articular injection of MIA, which mimic exactly human OA. In this study, the effectiveness of PJ in improvement of histopathological damages is emphasized and its chondroprotective effect in vivo is highlighted.

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