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Information Seminar Paper

Title

IN VITRO EFFECT OF AQUEOUS EXTRACT RUTA GRAVEOLENS L. ON HUMAN SPERM

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Abstract

 Background: One of the contraceptive plants which have been introduced in Iranian traditional medicine is Ruta graveolens L. It had been used as a vaginal contraception. To find out its contraceptive effect, we experienced its aqueous and boiled aqueous extract on human sperm in vitro.Design: Different amounts of lyophilized extract of Ruta graveolens L. were added to an amount of fresh semen, containing 106 cells in a 1:1 volumic ratio. Motility, viability, morphology of cells, and sperm revival tests were assessed according to WHO parameters. Osmolarity and pH of extracts were also figured out.Results: The sperm immobilization effects of the aqueous extract appeared immediately in a dose-dependent manner and 100% of the sperms became immotile at a concentration of 100 mg/ml but viability of cells was intact. After washing the sperms, motility returned in 30.8±3.2 of the sperms, besides coiled tails in 38.6±5.5% of them (p=0.001). The part of the extract, responsible for immobilization was stable upon boiling. The extracts were hyperosmolar and had an acidic PH.Conclusion: Ruta graveolens L. aqueous extract contains some thermostable chemical compounds that could immobilize human sperm cell in a dose dependent manner, without being toxic for cells. High Osmolarity and low pH are two other motility inhibitors, so it seems that immobilization effect of this extract is multifactorial. It could be promising as a new natural contraceptive agent.

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