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Information Seminar Paper

Title

MORPHINE ADMINISTRATION INCREASES THE DENSITY OF N-TYPE VOLTAGE-DEPENDENT CALCIUM CHANNELS AND CHANGES THEIR SPLICE VARIANT'S EXPRESSION PATTERN IN RAT HIPPOCAMPUS AND LUMBAR SPINAL CHORD

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Abstract

 Introduction: Functional and structural diversity among voltage-gated calcium channels is an important mechanism employed by cells to optimize calcium dependent signaling. The Cav2.2 gene which encodes the functional core of the N-type calcium channels (a1B( has the potential to generate several Cav2.2 splice variants with different properties. However the functional significance of most of these splices variants and their distribution through the CNS has not been established.Methods: After removal of hippocampus and lumbar spinal chord, we extracted total RNA and performed RT-PCR reaction. Resulted cDNA was used as template in the PCR reaction.Results: By RT-PCR method we could detect 6 splice variants of a1B which two of them are novel and had not been reported in rat before. Besides we found that morphine administration increases calcium channel density and changes the expression pattern of a1B splice variants and produces new variants that have not been detected in the intact animals.Conclusion: These findings can help us to understand better the molecular mechanism of morphine in CNS and the tolerance which it causes.

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