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Information Seminar Paper

Title

THE DETERMINATION OF VALPROIC ACID PLASMA LEVEL IN IRANIAN EPILEPTIC PATIENTS

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Abstract

 Valproic acid (2-propylpentanoic acid, VPA) has been widely used in the treatment of a variety of seizure types as major antiepileptic drugs. The determination of antiepileptic drugs in body fluids on a routine basis is important to clarify and control their therapeutic and toxic effects and to assess the patient's compliance to therapy. A simple, reproducible and rapid gas chromatography method for precise determination of valproic acid (VPA) in human plasma has been developed. Total time for sample preparation and analysis step is less than 30 minutes. After protein precipitation valproic acid (VPA) was extracted into chloroform phase. By direct injection of this phase on to Stabilwax®-DA capillary GC column, symmetrical chromatographic peak was obtained without the need for derivatization. The calibration curve was proved to be linear (r2=0.9994) in a wide concentration rang (0.5-150 mg/ml) Inter-day and Intra-day accuracy and precision of this method was investigated during the method validation. The method was shown to be highly reproducible with a limit of detection (LOD) 400ng/ml of VPA in human plasma.

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