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Title

IN VITRO NEUROTOXICITY OF DIAZINON ON PC12 AND SKNMC CELL LINES

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Abstract

 Introduction: Diazinon is one of the organophosphorate insectisides which is used widely in our country for agricultural purposes. Many studies have reported the neurotoxicity of this insecticide but we don't know whether it differs in different cell lines or not. In the present study the influence of various Diazinon dilutions on two neuronal cell lines (PC13 & SKNMC) was assessed.Method: Cells were cultured in DMEM medium containing 10% fetal bovine serum, 100 u/ml penicillin plus 100µg/ml streptomycin. The cells were exposed to various Diazinon dilutions for 72 hours then the growth rate was evaluated by MTT-assay.Results: The SKNMC cell growth in 1,10, 25, 50 & 100 mg/ml dilutions were decreased by 8.5%, 27.18%, 62%, 89.66% & 98.5% respectively.(IC50=16.59). The statistically significant reduction of cell growth was observed in the exposure of 10 g/ml and higher dilutions of Diazinon (p<0.05). The PC12  cells growth in1, 10, 25, 50 & 100 g/ml dilutions were decreased by 1%, 2%, 27.5%, 60.25% & 87.66%.The cell growth significantly decreased in dilutions higher than 25 mg/ml (p<0.05). Diazinon IC50 for PC12 & SKNMC was 38.17 & 16.59 mg/ml.Conclusion: The findings indicate that SKNMC cells are more sensitive to Diazinon than PC12 cells, so it suggests that Diazinon has differential toxic effects on different neuronal cell lines.

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