LEISHMANIA SP. DNA DETECTING IN CUTANEOUS PARAFFIN EMBEDDED BLOCK BY A NOVEL HEMI-NESTED POLYMERASE CHAIN REACTION METHOD

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MORAVEJ HOSSEIN; DOUSTI ABAS; VESAL A.; HOSSEINZADEH -; KASEMI B.; ADELI AHMAD; GOMARI H.; MAHBOUDI FEREYDOUN

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Seminar Paper

Paper Information

Seminar: NATIONAL CONGRESS OF BIOTECHNOLOGY OF IRAN
Year:2005 | Issue:4

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Title

LEISHMANIA SP. DNA DETECTING IN CUTANEOUS PARAFFIN EMBEDDED BLOCK BY A NOVEL HEMI-NESTED POLYMERASE CHAIN REACTION METHOD

Author(s)

Keywords

HEMI-NESTED PCR

LEISHMANIA

PARAFFIN EMBEDDED BLOCK

Abstract

To investigate Leishmania Sp. DNA in cutaneous paraffin embedded blocks, a hemi-nested PCR method was developed and tested five cutaneous paraffin blokes which suspected to Leishmania Sp. infection. DNA form paraffin blocks were extracted by DNP™ DNA Extraction kit and then subjected for PCR and sequencing. An internal control was carried out that determined human factor XIII genome to investigate accuracy of DNA extraction. Three of five samples showed positive Leishmania DNA amplification. Results of sequencing showed the Leishmania genome sequences in all three positive samples. In conclusion, the developed hemi-nested PCR method can readily detect Leishmania DNA in the paraffin embedded samples that are suspected to be infected with Leishmania.

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APA: Copy

MORAVEJ, HOSSEIN, DOUSTI, ABAS, VESAL, A., HOSSEINZADEH, -, KASEMI, B., ADELI, AHMAD, GOMARI, H., & MAHBOUDI, FEREYDOUN. (2005). LEISHMANIA SP. DNA DETECTING IN CUTANEOUS PARAFFIN EMBEDDED BLOCK BY A NOVEL HEMI-NESTED POLYMERASE CHAIN REACTION METHOD. NATIONAL CONGRESS OF BIOTECHNOLOGY OF IRAN. SID. https://sid.ir/paper/902780/en

Vancouver: Copy

MORAVEJ HOSSEIN, DOUSTI ABAS, VESAL A., HOSSEINZADEH -, KASEMI B., ADELI AHMAD, GOMARI H., MAHBOUDI FEREYDOUN. LEISHMANIA SP. DNA DETECTING IN CUTANEOUS PARAFFIN EMBEDDED BLOCK BY A NOVEL HEMI-NESTED POLYMERASE CHAIN REACTION METHOD. 2005. Available from: https://sid.ir/paper/902780/en

IEEE: Copy

HOSSEIN MORAVEJ, ABAS DOUSTI, A. VESAL, - HOSSEINZADEH, B. KASEMI, AHMAD ADELI, H. GOMARI, and FEREYDOUN MAHBOUDI, “LEISHMANIA SP. DNA DETECTING IN CUTANEOUS PARAFFIN EMBEDDED BLOCK BY A NOVEL HEMI-NESTED POLYMERASE CHAIN REACTION METHOD,” presented at the NATIONAL CONGRESS OF BIOTECHNOLOGY OF IRAN. 2005, [Online]. Available: https://sid.ir/paper/902780/en

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