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Title

ANTIBODY SCREENING AND IDENTIFICATION BY GEL METHOD IN THALASSEMIC PATIENTS

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 Start Page 99 | End Page 108

Abstract

 Background and Objectives: Thalassemic patients need regular BLOOD TRANSFUSION because of chronic nature of disease. Since just major blood groups are controlled in compatibility tests, formation of antibody against minor blood groups (irregular antibodies) can cause transfusion reactions (delayed hemolytic reaction) and may produce complications for patients. In this study, we decided to perform SCREENING and typing of antibodies with gel method and tried to substitute this modern method for routine tests.Materials and Methods: In a descriptive study 441 THALASSEMIA patients having referred to two thalassemic centers in Tehran and Ghazvin were studied. Data forms about BLOOD TRANSFUSION status were filled out for all patients from whom blood samples were then taken. SCREENING antibody test with tube method was performed for all patients followed by assessment with gel method according to its standard pattern and respective program. Antibody identification test was performed according to the respective program as well. Results of obtained data from forms filled out by patients were assessed with SPSS 11.5 software; chi-square test was done with 95 % confidence interval.Results: 441 patients consisting of 234 males (53.1%) and 207 females (46.9 % ) with the mean age of 22.6 years (SD ± 9.27) participated in the study. Out of the total number of subjects, there are 362 THALASSEMIA major (82.1%) and 79 THALASSEMIA intermedia (17.9%). SCREENING showed 391 patients (88.7%) without versus 50 (11.3) with alloantibodies. From among identified antibodies, anti-Kell and anti-D were present in 28% and 16% of cases respectively; antibodies to other Rh subgroups including c, C, e, E and Cw appeared in 26% of cases. Comparing results of microtube gel method with tube method for SCREENING shows that 26 patients recognized as positive in gel method were negative in tube method.Conclusions: Regarding the alloimmunization frequency in thalassemic patients, it is suggested that RBC phenotype especially subgroups of Rh, Kell, Kidd, and Duffy be checked (in addition to ABO Rh(D) group) for every newly diagnosed THALASSEMIA patient; it is further recommended that in every crossmatch for BLOOD TRANSFUSION. Blood bag be selected based on Rh subgroups and Kell in conformity with patient phenotype; this would decrease alloimmunization by 90%.

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