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Title

MOLECULAR DIAGNOSIS OF B CELL NON-HODGKIN LYMPHOMA BY EVALUATION OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT

Pages

 Start Page 157 | End Page 166

Abstract

 Background and Objectives: Rearrangement of V, D, and J segments of IMMUNOGLOBULIN heavy chain gene with inserted or deleted nucleotides within rearranged segments makes unique hypervariable regions (CDR-3). These regions can be used for evaluation of B cell clonality for the purpose of molecular diagnosis of NON-HODGKIN LYMPHOMA (NHL) and for confirmatory diagnosis in suspicious cases.Materials and Methods: In this study, samples of 42 patients were collected from Taleghani, Baqhiyatalah, and Aliasghar hospitals; out of this number, there were 22 patients with diagnosis of B cell NHL, 10 with reactive hyperplasia, and 10 with malignant lymphoma. After DNA extraction from formalin fixed paraffin embedded tissues, PCR was done using consensus primers for amplification of CDR-3 region. PCR products were analyzed after heteroduplex analysis using polyacrylamide gel electrophoresis and silver stain.Results: Clonal patterns in group 1 (B cell NHL), 2 (reactive and follicular hyperplasia), and 3 (morphological diagnosis without immunohistochemistry) were observed in 77.2%, 0%, and 70% of patients, respectively.Conclusions: Our findings are compatible with other international studies with minor differences. The diagnosis of B-cell lymphoid malignancy can frequently be substantiated by detecting clonal IMMUNOGLOBULIN heavy chain (IGH) GENE REARRANGEMENT.

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