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Title

THE IMPACT OF STRESS DURING PREGNANCY ON CHILDREN’S’ SEIZURET AND DEVELOPING HIPPOCAMPUS IN MICE OFFSPRINGS

Pages

 Start Page 333 | End Page 345

Abstract

 Background & aim: The effect of STRESS on the nervous system activity may be due to alterations in the structure of the nervous system. In this study, the effect of maternal STRESS was examined on the SEIZURE THRESHOLD and hippocampal structure of their offspring during PREGNANCY.Methods: In the present study, thirty pregnant mice were divided into two groups. The No STRESS group and STRESS group which received one hour immobilization STRESS from 1 to 14 day of PREGNANCY. The SEIZURE THRESHOLD test was performed on offspring by injection of Pantilen tetrazol (PTZ). To study the HIPPOCAMPUS development, the mice offsprings were divided into three groups: The control group mother received no immobilization STRESS and their offspring also received no PTZ. The Sham group received no immobilization STRESS on the pregnant rat but their offspring received PTZ. But in experimental group, the pregnant rat received immobilization STRESS and their offspring received PTZ too. At the end of the experiences, all offspring’s were killed by chloroform and their HIPPOCAMPUS was fixed in boein solution. After processing a 5μm sections were prepared and stained by hematoxylin and eosin. The pyramidal and granular layers thickness in the HIPPOCAMPUS were measured using Motic software and the number of cells in these layers and the number of blood vessels in the molecular and polymorphic layers were counted. The data were analyzed using ANOVA and T-test.Results: a significant increase in SEIZURE THRESHOLD was observed in offsprings whose mothers were under STRESS compared with offspring whose mothers were not under STRESS (p<0.001). A significant increase in average thickness of hippocampal pyramidal and granular in the experimental group was observed compare with the control group (p<0.001). In addition, the mean thickness of pyramidal and granular layers of HIPPOCAMPUS significantly increased in the experimental group compared to control group. The number of cells in hippocampal and granular layers significantly decreased in the experimental group when compared with control and sham groups (p<0.001). Compared with the sham and control groups a significant reduction was observed in granular hippocampal pyramidal cells layers of different areas of experimental group (p <0.001).Conclusion: Prenatal STRESS can cause an increase in SEIZURE THRESHOLD and also impair offspring' s development and their hippocampal structure.

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