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Effect of IL-2 co-expressed or co-inoculated with immunodominant epitopes from VP1 protein of FMD virus on immune responses in BALB/c mice




 Objective(s): The results of studies on vaccine development for Foot-and-mouth disease (FMD) virus show that the use of inactivated vaccines for FMD virus is not completely effective. Novel vaccinations based on immuno-dominant epitopes have been shown to induce Immune responses. Furthermore, for safety of immunization, access to efficient Adjuvants against FMD virus seems to be critical. Materials and Methods: In this study, we produced epitope recombinant vaccines from the VP1 protein of the FMD virus for serotype O of Iran. Constructs were included polytope (tandem-repeat multipleepitope), polytope coupled with Interleukin-2 (polytope-IL 2) as a molecular Adjuvant and IL-2. Three expression vectors were constructed and expressed in Escherichia coli BL21 (DE3). To evaluate whether these recombinant vaccines induce Immune responses, BALB/c mice were injected with the recombinant vaccines and their Immune responses were compared with a negative control group. The humoral and cellular Immune responses were measured by ELISA. Results: The results showed that IL-2 co-expressed or co-inoculated with Polytope protein enhances the immune effect of multiple epitope recombinant vaccine against FMD virus. The results of total immunoglobulin G (IgG), IgG1, and IgG2a levels and secretion of interferon gamma (IFN-γ ), IL-4 and IL-10 revealed that there were significant differences between negative control group and other injected mice with the recombinant vaccines (P<0. 05). Conclusion: Observations indicated that the epitope recombinant plasmid of the VP1 protein coexpressed or co-inoculated with IL-2 was effective in inducing an enhanced Immune response. Therefore, IL-2 can be recommended as a potential Adjuvant for epitope recombinant vaccine of the VP1 protein from FMD virus.


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