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Title

Effect of genistein on expression of pancreatic SIRT1, inflammatory cytokines and histological changes in ovariectomized diabetic rat

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 Start Page 423 | End Page 429

Abstract

Genistein is reported to have anti-diabetic and anti-inflammatory functions, in particular, direct effects on β-cell proliferation and insulin secretion. In this study, we investigated the anti-inflammatory effect of Genistein on the pancreatic β-cells in ovariectomized diabetic rat. Materials and Methods: Forty female rats were divided into four groups: sham, bilateral ovariectomy (OVX), OVX. D (OVX+Diabetes) and OVX. D. G (OVX. D+Genistein). After bilateral ovariectomy, rats in the diabetic groups were fed high-fat diet (HFD), ad libitum for 4 weeks, and then a low dose of streptozotocin (STZ) (30 mg/kg) injected intraperitoneally. Genistein (1 mg/kg/day; SC) was administrated for 8 weeks. At the end of 8 weeks, pancreas tissue was removed and used for western blotting and Hematoxylin-Eosin staining. Results: Treatment with Genistein declined inflammation and tissue injury, and this decline was correlated with the expression of SIRT1. OVX and OVX. D significantly increased Nf-к B and IL-1β expression and decreased SIRT1 levels compared to sham group (P<0. 05). Significant reduction of Nf-к B and IL-1β , and increasing of SIRT1 were observed during Genistein treatment (P<0. 05). Conclusion: Estrogen deficiency alone or with HFD increased pancreatic inflammation. However, subcutaneous administration of gtenistein prevented from these inflammatory changes in the pancreas of a surgery animal model of ovariectomy with or without Diabetes. Our results support the potential preventing effect of Genistein from pancreatic injury.

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