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Title

STUDY ON PRODUCING POSSIBILITY OF AMINO ACIDS FROM DATE PALM WASTES BY TWO MUTANT CORYNEBACTERIUM GLUTAMICUM CECT690 & CECT77

Pages

 Start Page 55 | End Page 64

Abstract

 Regarding the vast applications of AMINO ACIDS such as GLUTAMIC ACID in food industry, chemistry, pharmacology and cosmetics on one and the effort to overcome the problem of DATE WASTES in Iran on the other hand, the production of AMINO ACIDS such as GLUTAMIC ACID from date fruit wastes was studied.Two mutants CORYNEBACTERIUM GLUTAMICUM CECT690 & CECT77 were used to study of possible producing of AMINO ACIDS from DATE WASTES. Results of date fruit wastes analysis indicated that these wastes are nutritious to bacterial growth. Since date fruit wastes are complex and unknown medium for bacterial growth, in this study some effective variables on production of the AMINO ACIDS using screen design (used as an experimental design) including 7 independent variables in 2 levels ( minimum and maximum) were investigated.Results of AMINO ACIDS measured by high performance liquid chromatography showed that THREONINE and GLUTAMIC ACID are produced more than other AMINO ACIDS.According to the statistical analysis, the most effective variables on GLUTAMIC ACID production are the amount of date fruit wastes, time of penicillin addition, phosphate amount and type of microorganism respectively and the least effective variables on GLUTAMIC ACID production are biotin amount, temperature and nitrogen source.Among interaction effects variables, interaction effect of DATE WASTES amount and time of penicillin addition is the most effective of variable on GLUTAMIC ACID production. Results of statistical analysis of THREONINE production showed that the most effective variables on THREONINE production are time of penicillin addition, the amount of date fruit wastes and type of microorganism.The results from HPLC also showed that many AMINO ACIDS such as alanine, valine, lysine, proline, tyrosine, phenylalanine, leucine and isoleucine a part from GLUTAMIC ACID and THREONINE are produced.

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