Journal Paper

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Journal: یاخته
Year:2017 | Volume: | Issue:
Start Page: | End Page:

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Title

CO-ADMINISTRATION OF VITAMIN E AND TESTOSTERONE ATTENUATES THE ATRAZINE-INDUCED TOXIC EFFECTS ON SPERM QUALITY AND TESTES IN RATS

Pages

 Start Page 292 | End Page 305

Abstract

 Objective: ATRAZINE (ATZ) as a widely used herbicide is considered as a potent endocrine disrupter which adversely affects reproductive systems in both genders. This study aimed to assess the effects of TESTOSTERONE (T) - and VITAMIN E (VitE) - alone and their co-administration on testicular function and sperm parameters after exposure to ATZ in rats.Materials and Methods: In this experimental study, the rats (n=30) are assigned into the following 5 groups: control-sham group (n=6) receiving corn oil, ATZ group (n=6) receiving 200 mg/kg ATZ alone, ATZ+VitE group (n=6) receiving 150 mg/kg ATZ+VitE, ATZ+T group (n=6) receiving 400 mg/kg ATZ+T, and ATZ+VitE+T group (n=6) receiving ATZ+VitE+T for 48 consecutive days. Total antioxidant capacity (TAC), total thiol molecules (TTM), and malondialdehyde (MDA) were analyzed. Serum levels of T, luteinizing hormone (LH), and inhibin-B (IN-B) were also determined. Histological examination and sperm analysis were performed. The data were analyzed using Graph-Pad Prism software version 2.01.Results: Co-administration of VitE and T significantly (P<0.05) increased ATZ-decreased TAC and TTM levels and reduced ATZ-increased MDA content. T and VitE significantly (P<0.05) increased serum levels of ATZ-reduced T (1.94±0.96), IN-B (122.10±24.33) and LH (0.40±0.10). The T+VitE animals showed a reduction in apoptotic cells and an increase in Leydig cells steroidogenesis. Co-administration of T and VitE significantly (P<0.05) reduced the ATZ-induced DNA disintegrity and chromatin de-condensation. VitE and T protected germinal cells RNA and protein contents against ATZ-induced damages.Conclusion: T and VitE in simultaneous form of administration were able to normalize the ATZ-induced derangements through promoting antioxidant capacity and endocrine function.

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