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Title

EXPRESSION ANALYSIS OF GATA4, TBX5 AND NKX2.5 GENES INVOLVED IN CONGENITAL HEART DISEASE

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Abstract

 Background: Congenital HEART DISEASE (CHD) is the most widespread congenital disease in newborn babies and is one of the main causes of death worldwide. The causal agent of heart congenital diseases is unknown but genetic factors have an important role in prevalence of disease.Objectives: The main objective of this research is comparison of the gene expression level of three GATA4, TBX5 and NKX2.5 genes in three groups of children between 6 months and 13 year old with congenital HEART DISEASE.Patients and Methods: In this case-control study, 30 samples from each cyanotic and acyanotic patients and 30 samples from healthy children as control were used. RNA extraction was done using commercial kit and gene expression analysis was performed by qRT-PCR approach in three replication using GATA4, TBX5 and NKX2.5 genes. Data analysis was done by REST software.Results: The results of RNA extraction and cDNA synthesis of all sample showed high quantity and quality of genetic materials. Expression level of tested genes was reduced in two patients group. In cyanotic group reduction was more than acyanotic samples. All tested gene were reduced in both group. TBX5 gene was suppressed more than other genes.Conclusions: Based on our results we could conclude that a gene family play an important role in cardiogenesis process and heart formation. These genes are closely related together. So a genetic consultation for such diseases on parents of these patients to determine the probable genetic mutations is recommended.

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