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Title

THE EFFECT OF PARA-NONYLPHENOL ON THE VIABILITY AND MORPHOLOGY OF RAT BONE MARROW MESENCHYMAL STEM CELLS

Pages

  1-11

Abstract

 Background: This study investigated the effect of para-nonylphenol as an environmental pollutant on the viability and MORPHOLOGY of the rat bone marrow MESENCHYMAL STEM CELLS.Materials and Methods: In this Experimental study, Under sterilized conditions, rat bone marrow MESENCHYMAL STEM CELLS were extracted in DMEM containing 15% FBS and Pen/Strep up to the third passage and then treated with 0, 10, 50, 100, 150, 200, and 250 mM of para-nonylphenol for 12, 24, 36, and 48 hrs. Viability of the cells was measured through trypan blue and MTT staining. Eventually, 100 mM dosage and 24 hrs of treatment were selected for carrying out the investigations. The cells MORPHOLOGY was studied using fluorescent dye (Hochest, propidium iodide, acridine orange, and monodensyl cadaverine) and the protein profile of the cells were studied using SDS-PAGE. Data were analyzed using one and two way ANOVA.Results: Based on the two-way ANOVA, cumulative effect of treatment time and dosage caused a highly significant reduction (P<0.001) in the viability of rat bone marrow MESENCHYMAL STEM CELLS. One way ANOVA indicated a significant difference in the viability of the cells from 100 mM of para-nonylphenol onwards through all treatment times in comparison to the control group (P<0.05).Condensation and deformation of the nuclei, membrane disruption, and presence of several vacuoles in the cytoplasm were also observed.Conclusion: Para-nonylphenol toxicity resulted in morphological changes in rat bone marrow MESENCHYMAL STEM CELLS. This reduction in the viability of the cells was dependent on dose and time.

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