Journal Paper

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Journal: یاخته
Year:2012 | Volume: | Issue:
Start Page: | End Page:

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Title

RGD MODIFIED PCL/GELATIN HYBRID NANOFIBER FOR POTENTIAL APPLICATIONS IN TISSUE ENGINEERING; POSTER PRESENTATION

Pages

 Start Page 55 | End Page 56

Keywords

PCL 
RGD 

Abstract

 Objective: Nanofibrous polycaprolactone (PCL) SCAFFOLDs have many advantages in biomedical applications, but its usefulness in tissue engineering is limited because of the lack of bioactivity. In this work, we developed a PCL/Gelatin hybrid nanofibrous SCAFFOLD which further functionalized by RGD-containing peptide as abiomimetic cell adhesion motif.Materials and Methods: Collagen-derived RGD-containing peptide with an Iodine tag was synthesized using standard Fmoc-chemistry on Rinkamide-MBHA resin. The peptide was cleaved by 95% TFA, precipitated by diethyl ether, lyophilized, and purified by Preparative HPLC. MALDI-TOF mass spectrometry was used for analyzing peptide mass. A solution of 10% (w/v) PCL and GELATIN (1: 1) was dissolved in trifluoroethanol, then PCL/Gelatin nanofibrous SCAFFOLD was fabricated by electrospinning. To improve performance and structural stability, electrospun SCAFFOLD was crosslinked by EDC. To develop hybrid, bioactive SCAFFOLD, RGD-containing peptide was immobilized on activated SCAFFOLD by the same strategy as crosslinking. Immobilization of peptide was assessed by UV absorbance of the peptide. The morphology of NANOFIBERs was characterized by SEM.Results: Successful peptide synthesis was confirmed by MALDI-TOF mass spectrometry with theoretically predicted mass of 1185.21. SEM images verified PCL/ GELATIN NANOFIBER fabrication with diameters between 300 to 400 nm which appeared in random network.  SEM images showed successful crosslinking of PCL/ GELATIN NANOFIBERs. UV spectrophotometry results indicated that the peptide has been immobilized on SCAFFOLD with a concentration of about 200 mg/cm2.Conclusion: The present study introduces a hybrid NANOFIBER SCAFFOLD that further functionalized by immobilization of RGD-containing peptide. This RGD-functionalized PCL/Gelatin NANOFIBER SCAFFOLD improves cell-biomaterial interaction and could have potential applications in tissue engineering and regenerative medicine.

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