Journal Paper

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Journal: یاخته
Year:2012 | Volume: | Issue:
Start Page: | End Page:

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Title

INVESTIGATION OF NEURAL GROWTH FACTOR GENE EXPRESSION IN UNRESTRICTED SOMATIC STEM CELLS; POSTER PRESENTATION

Pages

 Start Page 47 | End Page 47

Keywords

NGF 

Abstract

 Objective: Delivery of neurotrophic factors to the site of injury may promote neural cells repair after peripheral or central injury, therefore the transfer of the stem cells producing this kind of factors could be an effective method with respect to injury repair. Unrestricted somatic stem cells (USSC) are non-hematopoietic stem cells isolated from the umbilical cord blood and are appropriate using in cell therapy. Different studies, in vitro and vivo models have shown the ability of these cells to support or trigger the neuronal/Axon regeneration. To identify the molecular mechanisms of this effect, the EXPRESSION of some neurotrophic factors such as BDNF and NT-3 have been investigated by different groups. Addressing the importance of the neural growth factor (NGF) in neuronal and axon regeneration, we investigated the EXPRESSION of NGF in USSCs in this study.Materials and Methods: Unrestricted somatic stem cells isolated from Umbilical cord blood, characterized by different markers, and cultured in DMEM with 10% FBS and 1% Penicillin- Streptomycin. Real time PCR and ELISA were used to determine the NGF EXPRESSION in USSCs and Co-culture was done to verify initial results.Results: Although real time PCR results showed the USSCs express NGF and some other neurotrophic factor genes such as BDNF and GDNF, ELISA results revealed that USSCs didn’t express NGF protein. Cell culture medium co-culture with PC-12 model cells showed that USSCs culture medium didn’t have any effect on PC-12 cells, as a NGF dependant cell line, and couldn’t induce differentiation in these cells.Conclusion: We conclude that USSCs don’t release functional NGF protein; therefore, USSC’s effects on neuronal regeneration induction would be related to the EXPRESSION of other trophic factor proteins such as BDNF, GDNF or NT-3.

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