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Title

MOLECULAR IDENTIFICATION AND DETECTION OF LYSIPHLEBUS FABARUM (HYM.:BRACONIDAE): A KEY PARASITOID OF APHIDS, BY USING POLYMERASE CHAIN REACTION

Pages

 Start Page 1453 | End Page 1463

Abstract

 All species in the genus Lysiphlebus Forster (Hymenoptera: Braconidae, Aphidiinae) are solitary endoparasitoids of aphids, which are one of the most difficult and unknown taxonomic groups to identify. A pair of species-specific primers as a MOLECULAR MARKER was designed to identify the most abundant and important APHID PARASITOID WASP, L. fabarum Marshall, collected from Guilan province (Iran). This primer pair, which produces a 148 bp fragment length, was developed for L. fabarum by using the ATPASE 6 gene region of mitochondrial DNA. The lower detection limit to amplify DNA of L. fabarumin a singleplex PCR was determined to be 72 pg of parasitoid DNA/ml PCR which was enough to detect this parasitoid in early life stages within its host. The L. fabarum eggs cannot be detected within Aphis fabae Scopoli (Hemiptera:Aphididae) earlier than 12 hours after oviposition. A pair of species specific primers designed for L. fabarum can be used to monitor the wasp populations under field conditions.

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