Journal Paper

Paper Information

Journal: یاخته
Year:2012 | Volume: | Issue:
Start Page: | End Page:

video

sound

Persian Version

View:

6,519

Download:

0

Cites:

Information Journal Paper

Title

MODULATION OF CXCR4 EXPRESSION ON CORD BLOOD STEM CELLS; POSTER PRESENTATION

Pages

 Start Page 66 | End Page 67

Keywords

Abstract

 Objective: CXCR4 is critical adhesion molecule in homing process. Modulation of its expression on CORD BLOOD (CB) CD34+could overcome delay following CORD BLOOD transplantation. Complicated network of growth factors including cytokines and neuropeptides in microenvironment has important role in regulation of this adhesion molecule. So, we aimed to assess the role of two neuropeptides Substance P (SP) and Calcitonin gene related peptide (CGRP) in addition to cytokine cocktail on CXCR4 expression.Materials and Methods: CD34+cells purified from CB were cultured in a serum-free liquid culture system. Different concentrations of SP and/or CGRP were used in combination with cytokine cocktail. Protein and genomic levels of CXCR4 was assessed by flowcytometry and real time RT-PCR.Results: Our data show increased CXCR4+CD34+ cells cultured with SP and/or CGRP by day 7. Concentration 10-9M either SP or CGRP increased the genomic expression of CXCR4 molecule by day 11 compare to control group.Conclusion: Our experiment indicates that SP and CGRP induce CXCR4 protein expression in 7 days culture and enhance its genomic expression. Consequently, over expression of CXCR4 improves engraftment of CB CD34+cells.

Cites

  • No record.
  • References

  • No record.
  • Related Journal Papers

  • No record.
  • Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops






    File Not Exists.