Journal Paper

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Journal: یاخته
Year:2011 | Volume: | Issue:
Start Page: | End Page:

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Title

STEM CELL AND REGENERATIVE MEDICINE: P-148: PPARγ1 EXPRESSION PROFILE DURING DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO DEFINITIVE ENDODERM

Pages

 Start Page 106 | End Page 107

Abstract

 Objective: The PPARs (peroxisome proliferatoractivated receptors) are members of the nuclear hormone receptor superfamily. Three major isoforms of PPARs (a, b and g) have been identified that each with a possibility of different ligands, target genes and biological roles.The expression of PPARa, b and g varies widely from tissue-to-tissue. PPAR g has been demonstrated to play an important role in the regulation of cell differentiation.PPAR g has 2 isoforms termed: g1, g2, which are arisen by differential transcription start sites and alternative splicing. In order to see the importance of PPAR g1 in differentiation process of embryonic stem cells, the present study was undertaken.Materials and Methods: Endodermal differentiation of mESCs (Royan B1 cell lines) cells was induced by culturing the cells in N2B27 media including for 5 days under treatment by Activin A at final concentration of 100 ng/ml with daily medium change. Treatment pursued by the same condition supplemented with retinoic acid (RA) (0.1 mM) in place of Activin A for more three days. In order to chase the mRNA level of PPAR g1, Total RNA was extracted from mESC, cells before RA treatment (d5) and after reaching to the endodermal cell fate (d8, DE) and cDNA synthesis was carried out to perform a real time quantification analysis for PPAR g1.Results: Despite a decrease in expression pattern of PPARg1 in d5 cells, the results revealed an increasing expression pattern of PPARg1 in DE differentiated cells (d8, DE) relative to mESC and d5, which was significant at p < 0.05.Conclusion: In the present research, we have attempted to have a further look into the expression level of PPAR g1 gene during the differentiation of stem cells to DEFINITIVE ENDODERM. In conclusion, our data clearly indicated that an increment in PPAR g1 upon RA treatment may be somehow is involved in regulation of stem cells differentiation in to DEFINITIVE ENDODERM which could be clarified in further studies.

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