PER, CTX-M, TEM AND SHV BETA-LACTAMASES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE ISOLATED FROM TEHRAN, IRAN

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NASEHI L.; SHAH CHERAGHI FERESHTEH; NIKBIN V.A.S.; NEMATZADEH SH.

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Journal: Iranian Journal of Basic Medical Sciences Year:2010 | Volume:13 | Issue:3 (46) Page(s): 111-118

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Title

PER, CTX-M, TEM AND SHV BETA-LACTAMASES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE ISOLATED FROM TEHRAN, IRAN

Author(s)

NASEHI L. | SHAH CHERAGHI FERESHTEH | NIKBIN V.A.S. | NEMATZADEH SH. | Issue Writer Certificate

Keywords

CTX-M PER

ESBLS

KLEBSIELLA PNEUMONIAE

SHV

TEM

Abstract

Objective(s): Different types of extended spectrum beta-lactamases (ESBLs) are encountered in the clinical settings worldwide. There are a few studies regarding the prevalence of ESBL genes among Klebsiella pneumonia isolates at Tehran especially those of blaPER and blaCTX. The aim of this study was to determine the prevalence of blaSHV, blaTEM, blaPER and blaCTX genes among clinical K. pneumoniae of different hospitals in Tehran.Materials and Methods: Two hundred isolates of K. pneumoniae were received from different clinical specimens. The susceptibility of the isolates to 10 different antibiotics was examined by disk diffusion test. The MICs for ceftazidime were also determined using micro-broth dilution assay. Isolates showing MIC³ 4 mg/ml for ceftazidime were screened for ESBL production by phenotypic confirmatory test (PCT) and subjected to PCR for studied genes. Variation among four amplified genes was evaluated using PCR-RFLP.Results: By disk diffusion test, resistance to ceftazidime and cefotaxime were 34.7% and 33.5% respectively. However, all strains were susceptible to imipenem. Eighty isolates showed MICs³4 mg/ml for ceftazidime of which 77 (96%) were positive for ESBL in PCT. The prevalence of blaSHV, blaCTX-M, blaTEM and blaPER among these isolates were 26%, 24.5%, 18% and 7.5%, respectively. No variation was detected in the genes by PCR-RFLP.Conclusion: As far as we know this is the first report of the blaPER-1 in K. pneumoniae in Iran. The blaCTX-M was the second most common gene detected among the ESBL positive isolates of K. pneumoniae. For rapid identification of ESBL producing isolates it was recommended that clinical laboratories adopt simple test based on CLSI recommendation for confirming ESBL production in enterobacterial species.

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APA: Copy

NASEHI, L., SHAH CHERAGHI, FERESHTEH, NIKBIN, V.A.S., & NEMATZADEH, SH.. (2010). PER, CTX-M, TEM AND SHV BETA-LACTAMASES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE ISOLATED FROM TEHRAN, IRAN. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 13(3 (46)), 111-118. SID. https://sid.ir/paper/571960/en

Vancouver: Copy

NASEHI L., SHAH CHERAGHI FERESHTEH, NIKBIN V.A.S., NEMATZADEH SH.. PER, CTX-M, TEM AND SHV BETA-LACTAMASES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE ISOLATED FROM TEHRAN, IRAN. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2010;13(3 (46)):111-118. Available from: https://sid.ir/paper/571960/en

IEEE: Copy

L. NASEHI, FERESHTEH SHAH CHERAGHI, V.A.S. NIKBIN, and SH. NEMATZADEH, “PER, CTX-M, TEM AND SHV BETA-LACTAMASES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE ISOLATED FROM TEHRAN, IRAN,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 13, no. 3 (46), pp. 111–118, 2010, [Online]. Available: https://sid.ir/paper/571960/en

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