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Title

STUDY OF HYPOGLYCEMIC EFFECT OF JUGLANS REGIA LEAVES AND ITS MECHANISM

Pages

 Start Page 57 | End Page 65

Abstract

 Background: Hypoglycemic effect of JUGLANS REGIA leaves was reported in other previous researches. Objective: To investigate the mechanism of hypoglycemic action of Julans regia Leaves Methanolic Extract (JRLME), we designed the following study.Methods: Male wistar rats were classified in five groups, each of six, including alloxan-induced diabetic rats treated with JRLME at doses of 250 (D+JRLMEa), 500 mg/kg (D+JRLMEb), Acarbose at dose 20 mg/kg (D+Ac), vehicle alone (DC) and normal rats treated with vehicle alone (NC). The Postprandial BLOOD GLUCOSE level was examined in a short time (after a single dose treatment) and a long time (after three weeks daily treatment) model. After three weeks of treatment, all groups’ animals were killed to dissect pancreatic and myocardium for RNA extraction and RT-PCR, to assess INSULIN and GLUT-4 genes expression, respectively. In addition, other normal rats were killed to extract intestinal brush border membrane for a-glucosidase solution preparation. The effect of JRLMEa on a-glucosidase activity was evaluated by an in vitro method, compared to Acarbose as a reference drug.Results: The plant extract had a significant hypoglycemic action in both short and long term models. Decrease in PBG level was the most at 8 hour after treatment and more in D+Ac (53%), D+JRLMEa (40%) and D+JRLMEb (29%) versus DC, respectively. There was also permanent PBG reduction in treated groups in comparison with the DC one during long term period.Conclusion: We did not find change in the INSULIN and GLUT-4 genes expression. On the other hand, the in vitro assay of a-glucosidase activity displayed inhibitory action of JRLME, like Acarbose, but less effectively.

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