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Title

GENE EXPRESSION OF CATALASE DURING NEURAL DIFFERENTIATION OF P19 CELLS

Pages

  100-101

Keywords

P19 

Abstract

 Objective: The P19 murine embryonal carcinoma (EC) cell line is a valuable in vitro model cell that can be differentiated into neurons by cellular aggregation in presence of the differentiating agent retinoic acid (RA).Materials and Methods: Total RNA from P19 cells was extracted and cDNA was synthesized. Using specific primers, respective cDNAs were amplified and were analyzed by semi-quantitative RT-PCR.Results: In this project, a peroxisomal gene such as CATALASE has been selected and the profile of its expression has been investigated in P19 cells. Expression of peroxisomal gene like CATALASE, as peroxisomal matrix protein in comparison with pluripotency markers such as OCT4 and Nanog, neural markers such as Pax6, Ngn-1, Map2 and a house keeping gene such as b-tubulin have been investigated by RT-PCR.Conclusion: Data indicated that during neural differentiation, expression of pluripotency markers have been down regulated while, expression of neural markers was significantly increased. However incase of CATALASE gene expression, there was an increase in CATALASE gene expression upon Retinoic acid treatment, during neural differentiation, which was observed at the final steps of NEUROGENESIS.

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    APA: Copy

    MOJBAFAN, M., GHAEDI, K., RAZAVI, S., TANHAEI, S., KARAMALI, F., RABIEI, F., & NASRESFAHANI, MOHAMMAD HOSSEIN. (2009). GENE EXPRESSION OF CATALASE DURING NEURAL DIFFERENTIATION OF P19 CELLS. CELL JOURNAL (YAKHTEH), 11(SUPPL. 1), 100-101. SID. https://sid.ir/paper/562964/en

    Vancouver: Copy

    MOJBAFAN M., GHAEDI K., RAZAVI S., TANHAEI S., KARAMALI F., RABIEI F., NASRESFAHANI MOHAMMAD HOSSEIN. GENE EXPRESSION OF CATALASE DURING NEURAL DIFFERENTIATION OF P19 CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2009;11(SUPPL. 1):100-101. Available from: https://sid.ir/paper/562964/en

    IEEE: Copy

    M. MOJBAFAN, K. GHAEDI, S. RAZAVI, S. TANHAEI, F. KARAMALI, F. RABIEI, and MOHAMMAD HOSSEIN NASRESFAHANI, “GENE EXPRESSION OF CATALASE DURING NEURAL DIFFERENTIATION OF P19 CELLS,” CELL JOURNAL (YAKHTEH), vol. 11, no. SUPPL. 1, pp. 100–101, 2009, [Online]. Available: https://sid.ir/paper/562964/en

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