Journal Paper

Paper Information

video

sound

Persian Version

View:

28,872

Download:

0

Cites:

Information Journal Paper

Title

STEM CELL AND REGENERATIVE MEDICINE: P-135: ACETONITRILE EXTRACT OF STREPTOMYCES CALVUS INDUCES GROWTH INHIBITION AND DIFFERENTIATION IN K562 CELL LINE

Pages

  100-100

Abstract

 Objective: Chronic myelogenous leukemia (CML) is a genetic disorder in pluripotent stem cell that acquires Philadelphia (Ph) chromosome in 95% of patients. At present, chemotherapy is the most important therapeutic manner for CML patients. Nevertheless, drug resistance and relapse of disease are the main challenges in CML therapy. To overcome this problem, it is necessary to screen new drugs especially from natural sources for cancer therapy. Recently, cytotoxic and anti-cancer activity of microbial secondary metabolites produced by STREPTOMYCES genous has been reported. In this study, we investigated the effects of acetonitrile extract isolated from STREPTOMYCES calvus fermentation broth in K562 cell line as a model of CML.Materials and Methods: K562 cell line was treated by various concentrations of acetonitrile extract (10- 1000 ng/ml) for 12, 24, 48 and 72 hours intervals. Antiproliferative effects of acetonitrile extract were studied by trypan blue exclusion test. Morphological changes in cells were evaluated by light microscope.Results: Acetonitrile extract induced growth inhibition and DIFFERENTIATION of K562 cells in a dose- and timedependent manner. For example, cell growth decreased 45-53% after 12 to 72 hours. In higher concentrations of the drug (700ng/ml and 1000 ng/ml) and after 72 hours treatment, some of the K562 cells develop a spindleshaped morphology and showed other morphological changes such as pseudopod formation suggesting differentiated K562 cells.Conclusion: According to growth inhibitory and DIFFERENTIATION effects of acetonitrile soluble metabolite of STREPTOMYCES calvus, this compound maybe a good candidate for treatment of CML patients in DIFFERENTIATION therapy approach.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    DEHNAD, A.R., GHANBARVAND, F., & MOUSAVI, M.A.. (2011). STEM CELL AND REGENERATIVE MEDICINE: P-135: ACETONITRILE EXTRACT OF STREPTOMYCES CALVUS INDUCES GROWTH INHIBITION AND DIFFERENTIATION IN K562 CELL LINE. CELL JOURNAL (YAKHTEH), 12(SUPPLEMENT 1 (THE 1ST INTERNATIONAL STUDENT CONGRESS ON CELL AND MOLECULAR MEDICINE)), 100-100. SID. https://sid.ir/paper/556927/en

    Vancouver: Copy

    DEHNAD A.R., GHANBARVAND F., MOUSAVI M.A.. STEM CELL AND REGENERATIVE MEDICINE: P-135: ACETONITRILE EXTRACT OF STREPTOMYCES CALVUS INDUCES GROWTH INHIBITION AND DIFFERENTIATION IN K562 CELL LINE. CELL JOURNAL (YAKHTEH)[Internet]. 2011;12(SUPPLEMENT 1 (THE 1ST INTERNATIONAL STUDENT CONGRESS ON CELL AND MOLECULAR MEDICINE)):100-100. Available from: https://sid.ir/paper/556927/en

    IEEE: Copy

    A.R. DEHNAD, F. GHANBARVAND, and M.A. MOUSAVI, “STEM CELL AND REGENERATIVE MEDICINE: P-135: ACETONITRILE EXTRACT OF STREPTOMYCES CALVUS INDUCES GROWTH INHIBITION AND DIFFERENTIATION IN K562 CELL LINE,” CELL JOURNAL (YAKHTEH), vol. 12, no. SUPPLEMENT 1 (THE 1ST INTERNATIONAL STUDENT CONGRESS ON CELL AND MOLECULAR MEDICINE), pp. 100–100, 2011, [Online]. Available: https://sid.ir/paper/556927/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    File Not Exists.