Journal Paper

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Journal: یاخته
Year:2010 | Volume: | Issue:
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Title

POSTER PRESENTATIONS; SODIUM ARSENITE IMPAIRS MINERALIZATION BY AFFECTING CALCIUM LEVEL AND ALKALINE PHOSPHATASE ACTIVITY IN DIFFERENTIATED RAT BONE MARROW MESENCHYMAL STEM CELLS

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 Start Page 31 | End Page 31

Abstract

 Objective: SODIUM ARSENITE is a well known toxic which is presented in drinking water and foods in polluted areas. The mutagenic property of this chemical has been determined and it is categorized in the class I carcinogen. Studies have shown that SODIUM ARSENITE causes DNA breakage and hypomethylation as well as hyper acetylation of histones. In addition, this environmental pollutant causes inactivation of enzymes specially those with SH group in their active site. To the best of our knowledge there is no data available to indicate the effect of SODIUM ARSENITE on differentiation of mesenchymal stem cells to osteoblasts. As the bone marrow mesenchymal stem cells are a very important source of osteoblasts generation thus the aim of this study was to investigate the effect of 25nM of SODIUM ARSENITE on differentiation of RAT BONE MARROW MESENCHYMAL STEM CELLS (BMCs) to osteoblasts.Materials and Methods: Using flash out technique under sterile condition, BMCs were extracted in the DMEM containing 15% of FBS and pen/strep then expanded till the 3rd passage and treated with osteogenic media (having dexametasone, ascorbic acid and b-glycerol phosphate) and 25 nM of SODIUM ARSENITE for 5, 10, 15 and 21 days. At the end of treatment periods, the cells were washed with tris buffere saline TBS (pH 7.4) then the CALCIUM concentration, and ALKALINE PHOSPHATASE acitivity of the cells were determined at 5, 10, 15 and 21 days of treatments. In addition, using qualitative alizarin red S estimation the level of MINERALIZATION of the differentiated cells was determined in treatment days. Furthermore, the morphology of the cells was studied using Hoechst, Propidium Iodide and Acridine Orange after 21 days of the treatment. Data was analyzed using one-way ANOVA and the p<0.05 was considered as the level of significance.Results: CALCIUM concentration reduced significantly (p<0.05) from the day 5 when cells treated with 25nM of SODIUM ARSENITE. Significant reduction of ALKALINE PHOSPHATASE activity was observed in the days 5, 10, 15 and 21 when cells treated with SODIUM ARSENITE. Result of this study showed that the MINERALIZATION in control group starts from the day 10 and its maximum was observed at day 21, while it significantly reduced (p<0.05) when the cells were treated with 25nM of SODIUM ARSENITE.Conclusion: As the results of this study showed, SODIUM ARSENITE impairs the MINERALIZATION process by reducing the CALCIUM level and ALKALINE PHOSPHATASE activity in differentiated BMCs. Thus exposure to this chemical might cause the reduction of MINERALIZATION which can be considered as the main reason of osteoporosis.

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