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Title

EXPRESSION OF PML TUMOR SUPPRESSOR IN A431 CELLS REDUCES CELLULAR GROWTH BY INHIBITING THE EGFR EXPRESSION

Writers

VALIAN S. | CHANG K.S.

Pages

 Start Page 57 | End Page 64

Abstract

 Our previous studies showed that the promyelocytic leukemia protein, PML, functions as a cellular and growth suppressor. Transient EXPRESSION of PML was also found to repress the activity of the epidermal growth factor receptor (EGFR) gene promoter. In this study, we have examined the effects of PML on A431 CELLS, which express a high level of EGFR protein. The PML gene was introduced into the cells using the adenovirus-mediated gene transfer system. Western blot analysis on the extracts from the cells expressing PML showed a significant repression in the EXPRESSION of the EGFR protein. The cells were examined for growth and DNA synthesis. The data showed a marked reduction in both growth and DNA synthesis rate in the cells expressing PML compared with the control cells. Furthermore, in comparison with the controls, the cells expressing PML were found to be more in G1 phase, fewer in S and about the same number in the G2/M phase. These data clearly demonstrated that the repression of EGFR EXPRESSION in A431 CELLS by PML was associated with inhibition of cell growth and alteration of the CELL CYCLE distribution, suggesting a novel mechanism for the known growth inhibitory effects of PML.

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