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Title

INVESTIGATION OF SENSITIVITY AND SPECIFICITY OF ACID PHOSPHATAESE TEST AND PROSTATE SPECIFIC ANTIGEN (PSA OR P30 ANTIGEN) IN IDENTIFICATION OF SEMINAL FLUID IN VAGINAL SWAB

Pages

 Start Page 187 | End Page 192

Keywords

PROSTATIC SPECIFIC ANTIGEN (PSA)Q3

Abstract

 Background & Aim: The existence or absence of the semen in the samples referred to forensic laboratory is detected by tests such as sperm-finding test, ACID PHOSPHATASE (AP) activity test, prostatic specific anti-gene (PSA, P30) test. Among them ACID PHOSPHATASE tests and PSA are the most applicable. In this study, the sensitivity and specificity of these two tests was examined and compared.Materials &Methods: This study includes two sections: first, vaginal swabs were obtained from 52female cases referred to infertility clinic for post coital test (as positive control group) and from 58 women who did not have intercourse for at least one week (negative control group) The samples from two groups, after their becoming dry, were referred to laboratory for acidphosphatase and PSA test in order to compare the results; second, vaginal swabs were obtained from 79 cases who have been referred to esfahan legal medic in center for investigation of sexual assault.Findings: The results suggest that the PSA sensitivity and specificity for semen detecting are quite effective. For the quantitative ACID PHOSPHATASE test (the result is recorded, precisely 2 minutes after adding substrate), the sensitivity rate of the method amounts to 88.4% and the specificity is determined 74.1 %. These two parameters for the qualitative phosphatase test in which the result is recorded after the exact 2 minutes, are found to be 98% for sensitivity and 5.1%for specificity.Conclusion: According to the study, utilizing the semi-quantitative phosphates test as a presumptive biomarker to discover semen cannot be reliable due to its high sensitivity (88.4%) and low specificity (74.1%). Therefore, in order to gain trustworthy results and to prevent false positive and negative reports another method should be used. We suggests that, according to the findings and the well-proven applicability of the PSA test as a definitive biomarker in detecting semen, Legal Medicine Organization of Iran (LMO) use this antigen in its laboratories as a confirmatory for the result of semi-quantitative acid phosphates test.

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