Journal Paper

Paper Information

video

sound

Persian Version

View:

119

Download:

0

Cites:

Information Journal Paper

Title

POLYMERASE CHAIN REACTION VERSUS CONVENTIONAL LABORATORY METHODS IN THE DIAGNOSIS OF FUNGAL KERATITIS

Pages

 Start Page 63 | End Page 67

Abstract

 Purpose: To compare the value of conventional laboratory methods and POLYMERASE CHAIN REACTION (PCR)in the diagnosis of FUNGAL KERATITIS.Methods: This cross sectional study was conducted at Khalili Hospital, Shiraz, Iran. Samples were taken from thirty-eight patients with findings suspicious for FUNGAL KERATITIS. Corneal scrapings were used for Gram, Giemsa and KOH stains, culture and PCR analysis.Results: Of 38 enrolled eyes, 25 eyes (68.5%) were judged to have FUNGAL infection based on positive cultures, staining, PCR or response to antifungal treatment. PCR detected fungi DNA in 17 of 25 samples (68% sensitivity). Staining (Gram, Giemsa and KOH) and culture yielded a positive result in 40% and 24% of samples respectively. Twenty one (84%) of 25 patients showed FUNGAL elements in at least one laboratory work up and 4 patients were diagnosed as FUNGAL KERATITIS only based on response to antifungal drugs. Conclusion: Compared to conventional laboratory methods, PCR based methods offer higher sensitivity and a faster diagnosis in FUNGAL KERATITIS.

Cites

  • No record.
  • References

  • No record.
  • Related Journal Papers

    Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops






    File Not Exists.