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Title

INVESTIGATION OF GENOTYPIC VARIATION AMONG PHYTOPHTHORA CAPSICI ISOLATES RECOVERED FROM DIFFERENT PROVINCES OF IRAN

Pages

 Start Page 65 | End Page 86

Abstract

 Phytophthora blight, crown and root rots caused by P. capsici are important diseases on many crops. In this investigation the population genetic structure of 29 representative isolates of a total 79 Iranian isolates of P. capsici, which showed the highest morphological diversity was studied using RAPD and ISSR primers. Five of 12 decamer RAPD primers as well as a 27 bp ISSR primer, exhibited reproducible polymorphic banding patterns. Cluster analysis using UPGMA method and Jaccard's coefficient showed that the RAPD primers all together categorize the isolates into two groups at 55% similarity. These two groups contained the southern (tropical) isolates including Fars and Khuzestan, 24% and central and northern (temperate) isolates, 76%. Cluster analysis of ISSR marker, divided the isolates into four groups at 40% similarity. The first group contained 17.5% and each of the other groups included 27.5% of isolates. The first group encompassed Mazandaran and Golestan isolates, the second contained Fars and Khuzestan isolates, Ardebil and East Azarbayejan isolates categorized in the third group and the fourth group included Tehran and Qazvin isolates. The results of banding patterns of ISSR and RAPD markers were analyzed together and the isolates categorized into five groups at 55% similarity. Mazandaran and Golestan isolates were in the first group, Fars and Khuzestan isolates in the second group, an isolate from Qazvin in third group, East Azarbayejan and Ardebil isolates in the fourth group and Tehran and Qazvin isolates in the fifth group. The frequencies of isolates in these five groups were 17.5, 24, 3.5, 27.5 and 27.5, respectively. This is the first report on the genetic diversity of Iranian POPULATIONS of P. capsici using RAPD and ISSR markers.

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