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Eco phytochemical, Ethnopharmacology, Antioxidant and anti bacterial activityin different extracts of Melilotus officinalis L. from Chaharbagh mountainous region-Semnan province


 Start Page 55 | End Page 63


 Melilotus officinalis L. is belongs to Fabaceae family, which has been used as a tonic, anti carminative, anti infection, anti diabetes and externally as a poultice for pains and ulcer wound. In many field observation the most ecological requirenments of M. officinalis L. and important experiences about traditional uses of this plant were obtained from rural healers. Different parts of plant (aerial parts in flowering stage, stem and root ) were collected from Charbagh Mountain (2300 msl) in May 2016. The ethanol and water extracts of plant parts were obtained by maceration method, Total phenol (TP) and flavonoid (TF) were estimated by spectrophotometry. The Antioxidant capacity was evaluated in vitro by the method of trapping the free radical DPPH, TAC and RP assay. The Antibacterial activity were obtained in vitro by using disc diffusion and the minimum inhibitory concentration (MIC) assay. According to the results aerial parts of this plant which has been used in this region as an anti infection, anti inflammation and tonic to treat of diabetes and arthritis. Phytochemical tests were showed that the ethanole extract of flowers had the highest total phenols concentration(38. 08± o. 13 mgGA/g), total flavonoids (62. 04 ± 0. 01 mgQU/g) and Antioxidant activity especially in DPPH radical scavenging with IC50=10. 61± 0. 81μ g/ml, in compare of BHA and BHT. The ethanole extract of flowers in 2300 m were showed the highest antimicrobial activity, especially against Staphylococus aureus, S. epidermaidis, Bacillus cereus and Entrococus faecalis L., respectively. In conclusion, there is the strong positive correlation between influence of extraction, plant parts on phytochemistry, Antibacterial and Antioxidant activity of M. officinalis L. and indicated that the ethanol exctract of flowers exhibited the highest Antioxidant and Antibacterial activity. So it can be used potentially as a good source of natural Antioxidant.


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