Journal Paper

Paper Information

Journal: کومش
Year:1393 | Volume:15 | Issue:3 (پیاپی 51)
Start Page:365 | End Page:371

video

sound

Persian Version

View:

360

Download:

0

Cites:

Information Journal Paper

Title

MOLECULAR IDENTIFICATION OF A 65 KDA MANNOPROTEIN ENCODED GENE OF CANDIDA SPECIES BY PCR

Pages

 Start Page 365 | End Page 371

Abstract

 Introduction: Invasive fungal infections represent a major public health concern. In particular, systemic candidiasis remains an increasing source of morbidity and mortality especially in immunocompromised patients such as neutropenic patients undergoing antiblastic chemotherapy or bone marrow transplants. Early diagnosis is often difficult.Consequently, effective treatment is often delayed. PCR has the potential to decrease the time required to diagnose fungal infections and therefore reduce the mortality associated with disseminated disease. The MP65 GENE of CANDIDA albicansis appropriate for detection and identification. The aim of this study was to identify different species of CANDIDA (C. albicans, C. glabrata, C. parapsilosis) with PCR technique.Materials and Methods: All 107 yeast isolates were identified on cornmeal agar supplemented with tween-80, germ tube formation in serum, and assimilation of carbon sources in the API 20 C AUX (Biomerieux, France). Then, all isolates were tesed by PCR using by different species-specific PCR primers selected within the MP65 GENE; a recently cloned gene encoding a mannoprotein adhesin.Results: A hundred of clinical isolates were detemined as C. albicans and 6 of the isolates detemined as C. glabrata and 1 of the isolates detemined as C. parapsilosis. The species-specific PCR primers allowed differentiation of each of three CANDIDA species by the amplicon length produced. The primers amplified all CANDIDA species DNA tested (100% positivity) giving a band of the expected length (475 bp for C. albicans, 361bp for C. glabrata, 124bp for C. parapsilosis). The results were agreed with all culture results for CANDIDA species detection.Conclusion: The results of this study showed that PCR method for rapid identification of CANDIDA species with specific primers is reproducible, simple and specific.

Cites

  • No record.
  • References

  • No record.
  • Related Journal Papers

    Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops






    File Not Exists.