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Title

INVESTIGATION OF ENZYME IMMOBILIZATION EFFECTS ON ITS CHARACTERISTICS

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 Start Page 183 | End Page 188

Abstract

 Background: Enzymes are well known as sensitive catalysts in the laboratory and industrial scale. To improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. Enzymes IMMOBILIZATION on solid supports such as epoxyfunctionalized ferric silica nanocomposite can be effective way to improve their characteristics.Methods: In this study silica coated magnetite nanoparticles were Functionalized with GPTSM as a linker, then IMMOBILIZATION reaction performed by using various amounts of lipase B from Candida Antarctica (CALB), for the next step IMMOBILIZATION effects on thermal stability and optimum pH were investigated in comparison with free CALB.Results: Results illustrated enzyme was successfully immobilized on nano particles and immobilized derivative retains 100% of its activity by 55oC while free CALB loss its activity at the same condition.Conclusion: IMMOBILIZATION of CALB on Fe3O4@SiO2 particles resulted in significant improvements in its characteristics such as thermal stability and methanol tolerance compared to the free CALB.

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