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Title

PRODUCTION OF MAG1 ANTIGEN OF TOXOPLASMA GONDII IN ESCHERICHIA COLI

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 Start Page 40 | End Page 44

Abstract

 Introduction: Toxoplasmosis is a parasitic infection caused by the protozoan TOXOPLASMA GONDII; it leads to serious medical problems in congenitally-infected and immunocompromised individuals, while it is quite harmless in immunocompetent individuals. Toxoplasma tissue cyst matrix protein (MAG1) induces early humoral and cell-mediated immune responses. Previous studies suggested recombinant MAG1 as a promising antigen for SERODIAGNOSIS of Toxoplasma infection.Methods: A DNA fragment encoding MAG1, comprising amino acids 50 to 207, was amplified from T. gondii RH strain and cloned in prokaryotic expression plasmid pET-15b (+). Recombinant MAG1 was expressed in ESCHERICHIA COLI, and highly purified in a single step by immobilized metal ion affinity chromatography.Results: The cloned DNA fragment was sequenced and showed 100% similarity with the published sequences available in GenBank Database. The results indicated proper antigenicity of recombinant MAG1. In Western blot analysis, purified protein showed a much stronger reactivity with sera from patients with acute Toxoplasma infection, compared to sera from chronic infection.Conclusion: MAG1 protein, alone or in combination with other acute-phase markers might be useful in discriminating acute/reactivated Toxoplasma infections from chronic forms.

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