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Title

COMPARATIVE STUDY OF RHODANESE KINETICS IN LIVER TISSUE

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 Start Page 174 | End Page 174

Abstract

 Rhodanese is an enzyme detoxifies cyanide by transfer of the reduced sulfur atom in a sulfane-containing compound to a thiophilic acceptor. This study was conducted to compare KINETICS of RHODANESE activity in sheep and chicken. To perform this study, LIVER tissues of both species were homogenized in sodium phosphate buffer and centrifuged in 400 g. The supernatant was precipitated with 70% ammonium sulfate and supernatant was used as the enzyme source. The enzyme activity was assayed in different concentration of thiosulfate and cyanide as substrate; then concentration of thiocyanate was determined by adding Golden stein’s reagent. Potassium thiocyanate was used to draw Standard curve. For thiocyanate Km values in sheep and chicken were 110.8 mM and 2285.7 mM respectively and Vmax values were 0.5 and 0.07 mM/S for sheep and chickens respectively. Km for potassium cyanate was 27.66 and 514.7 mM in sheep and chicken respectively. Vmax was calculated 0.82 mM and 0.063 for sheep and chicken respectively. In conclusion RHODANESE is less active in chicken than sheep.

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