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Information Journal Paper

Title

ALPHA-AMYLASE PRODUCTION BY SOME STARCH ASSIMILATING BACILLUS SPECIES

Pages

  150-150

Abstract

 ALPHA-AMYLASEs are STARCH-degrading enzymes which catalyze the hydrolysis of internal a-1, 4-O-glycosidic bonds in polysaccharides. These enzymes have many applications in food, paper, textile and pharmaceutical industries. About 30 % of world enzyme production market is provided by this enzyme. Alpha amylases are produced by plants, animals and microorganisms. However, microbial sources are the most preferred one for large scale production. Among microorganisms, ALPHA-AMYLASE is mainly derived from BACILLUS genus for commercial applications. Therefore, in this study, 13 different strains of BACILLUS genus were tested for their ability to hydrolyze and consume STARCH. Comparative studies were performed on the strains which were capable to consume STARCH. The curve of STARCH consumption by applying logul's solution was depicted for strains. The enzymes activity was assayed by using DNS (3, 5-dinitrosalicylic acid) at various times and temperatures. The best strain according to the enzymatic features was selected. Enzymatic extract was achieved by centrifugation. As ALPHA-AMYLASE was precipitated by ammonium sulphate, desalting process was performed by dialysis. Then ZYMOGRAPHY and determination of molecular weight for ALPHA-AMYLASE were performed. The best strain regard to enzymatic features was BACILLUS amyloliquefaciens. Maximum production of this enzyme was after 36 h. The enzyme activity was at the highest level at 70oC. Molecular weight of the enzyme was about 55KD. In conclusion, the selected strain can be an appropriate candidate for industrial production of ALPHA-AMYLASE.

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    APA: Copy

    GHOLAMZADEH, MINOO, EMTIAZI, GITI, & MOHABATKAR, HASAN. (2014). ALPHA-AMYLASE PRODUCTION BY SOME STARCH ASSIMILATING BACILLUS SPECIES. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, 3(SUPPL. (1)), 150-150. SID. https://sid.ir/paper/335883/en

    Vancouver: Copy

    GHOLAMZADEH MINOO, EMTIAZI GITI, MOHABATKAR HASAN. ALPHA-AMYLASE PRODUCTION BY SOME STARCH ASSIMILATING BACILLUS SPECIES. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS[Internet]. 2014;3(SUPPL. (1)):150-150. Available from: https://sid.ir/paper/335883/en

    IEEE: Copy

    MINOO GHOLAMZADEH, GITI EMTIAZI, and HASAN MOHABATKAR, “ALPHA-AMYLASE PRODUCTION BY SOME STARCH ASSIMILATING BACILLUS SPECIES,” MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 3, no. SUPPL. (1), pp. 150–150, 2014, [Online]. Available: https://sid.ir/paper/335883/en

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