video

sound

Persian Version

View:

41,646

Download:

29,561

Cites:

Information Journal Paper

Title

Silver Nanoparticle Trypsin Corona Formation and the Impacts on Enzymatic Potency

Pages

  241-250

Abstract

 Herein, the Protein corona formation on the spherical metal nanoparticles is studied to investigate the possible effects of Silver nanoparticles (AgNPs) on the protein activity and conformation. The digestion capability of Trypsin was monitored on the Human serum albumin (HSA) at standard enzymatic hydrolysis conditions in the absence and presence of different concentrations of AgNPs. So, the ratio of enzyme: HSA, the duration and the temperature of nanoparticle treatment were evaluated. The activity of treated Trypsin molecules, in the form of hard (HC) and soft corona (SC) was studied using sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE), and nano liquid chromatography electrospray (ion trap) mass spectrometry (nano LC-ESI/MS, LC-ESI/MS). In addition, the characteristics of Silver nanoparticles and the formation of HSA corona on the nanoparticle surface were monitored using ultra-violet/visible spectroscopy (UV-Vis), Dynamic light scattering (DLS) and fluorescence spectrophotometry. The results demonstrated that not only the corona formation but also the AgNPs/Trypsin interaction decreases the hydrolysis potency of Trypsin. Furthermore, interaction of AgNPs/HSA could influence both nanoparticles and HSA molecule features, companied with fluorescence study, that the HSA secondary structure. Also, LC-ESI/MS data revealed the most affected HSA triptics have α-helix structure.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    Darvishi Ganji, K., MIRZAJANI, F., ALIAHMADI, A., Fakhari Zavareh, A., & GHASSEMPOUR, A.. (2020). Silver Nanoparticle Trypsin Corona Formation and the Impacts on Enzymatic Potency. PHYSICAL CHEMISTRY RESEARCH, 8(2), 241-250. SID. https://sid.ir/paper/333941/en

    Vancouver: Copy

    Darvishi Ganji K., MIRZAJANI F., ALIAHMADI A., Fakhari Zavareh A., GHASSEMPOUR A.. Silver Nanoparticle Trypsin Corona Formation and the Impacts on Enzymatic Potency. PHYSICAL CHEMISTRY RESEARCH[Internet]. 2020;8(2):241-250. Available from: https://sid.ir/paper/333941/en

    IEEE: Copy

    K. Darvishi Ganji, F. MIRZAJANI, A. ALIAHMADI, A. Fakhari Zavareh, and A. GHASSEMPOUR, “Silver Nanoparticle Trypsin Corona Formation and the Impacts on Enzymatic Potency,” PHYSICAL CHEMISTRY RESEARCH, vol. 8, no. 2, pp. 241–250, 2020, [Online]. Available: https://sid.ir/paper/333941/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops