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Title

HIGH-FREQUENCY IN VITRO DIRECT SHOOT REGENERATION FROM NODAL EXPLANTS OF HYSSOP PLANT (HYSSOPUS OFFICINALIS L.)

Pages

 Start Page 187 | End Page 193

Abstract

 Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitroculture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11mmol) of TDZ and BAP in combination with 1 mmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01). In BAP treatments, the maximum shoot-buds induction (9 shoot-buds per explant) and shoot regeneration percentage (96.66%) were observed on MS medium fortified with 2.2 and 4.4 mmol BAP in combination with 1 mmol of IAA. In TDZ treatments, the highest regeneration percentage was achieved in MS medium supplemented with TDZ (2.2 mmol) and IAA (1μmol), and the maximum shoot-buds induction (19.83 shoot-buds per explant) was observed in medium containing 4.4 mmol of TDZ in combination with 1 mmol of IAA. The highest root production frequency (89.5%) was achieved in medium contained 9.84 mmol of IBA. Rooted plants were acclimatized successfully in greenhouse conditions with 100% survival. The protocol described here could be applicable for massin vitro production of the valuable MEDICINAL PLANTHyssopus officinalis L. for its genetic resource conservation as well as pharmaceutical purpose.

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