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Title

CLONE PURIFICATION, CHARACTERIZATION AND STANDARDIZATION OF LASOTA STRAIN FOR DEVELOPING A LIVE VACCINE AGAINST NEWCASTLE DISEASE VIRUS

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 Start Page 12 | End Page 19

Abstract

 Background and Aims: Newcastle disease (ND) is one of the most serious illnesses of chickens. LIVE VACCINEs are widely used to prevent chicken from the disease all over the world.Materials and Methods: To access the effective and potentiate ND vaccine, a homogenous subpopulation from LASOTA strain was selected following cultivation of the virus on primary chicken embryofibroblast (CEF) cells. Pathogenicity indices and molecular characterization of a several plaques at 3rd passage were analyzed and then the selected clone was candidate for vaccine development. ND vaccine was prepared according to the standard protocols.Results: The immunogenicity of the LIVE VACCINE was examined in specific pathogen free (SPF) and commercial chickens. The geometric mean hemagglutination-inhibition (HI) titers induced in chickens vaccinated with the cloned vaccine were not significantly differ than those induced in chickens vaccinated with the similar ND clone vaccine.Conclusion: Efficacy of the ND vaccine was estimated against the virus challenge. The results indicate that the cloned vaccine could confer a complete protection against NDV.

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