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Information Journal Paper

Title

SPECULAR MICROSCOPIC FEATURES OF CORNEAL ENDOTHELIAL VACUOLATION

Pages

  5-7

Abstract

 Purpose: To introduce a specular microscopic reference image for endothelial vacuolation in donated CORNEAs. Methods: Two CORNEAs from a donor with diffuse, round to oval dark areas at the endothelial level on slit lamp biomicroscopy and one normal-appearing donor CORNEA underwent SPECULAR MICROSCOPY, histopathologic evaluation and transmission electron microscopy. Results: SPECULAR MICROSCOPY of the two CORNEAs with abnormal-looking ENDOTHELIUM revealed large numbers of dark, round to oval structures within the ENDOTHELIUM in favor of endothelial vacuolation. Light microscopy disclosed variable sized cyst-like structures within the cytoplasm. Transmission electron microscopy showed electronlucent and relatively large-sized intracytoplasmic VACUOLEs. These features were not observed in the ENDOTHELIUM of the normal CORNEA. Conclusion: The specular microscopic features of endothelial vacuolation in donated CORNEAs were confirmed by light microscopy and transmission electron microscopy, therefore the specular image may be proposed as a reference to eye banks.

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