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Information Journal Paper

Title

EVALUATION OF PERIODONTAL LIGAMENT CELL VIABILITY IN THREE DIFFERENT STORAGE MEDIA: AN IN VITRO STUDY

Pages

  524-531

Abstract

 Objectives: This study was undertaken to evaluate the viability of PERIODONTAL LIGAMENT (PDL) cells of avulsed teeth in three different storage media.Materials and Methods: Forty-five premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups based on storage media used [Group I: milk (control), Group II: aloe vera (experimental), Group III: egg white (experimental)]. Following extractions, the teeth were placed in one of the three differ-ent storage media for 30 minutes, following which the scrapings of the PDL from these teeth were collected in Falcon tubes containing collagenase enzyme in 2.5 mL of phos-phate buffered saline. The tubes were subsequently incubated for 30 minutes and cen-trifuged for five minutes at 800 rpm. The obtained PDL cells were stained with Trypan Blue and were observed under optical microscope. The percentage of viable cells was calculated.Results: Aloe vera showed the highest percentage of viable cells (114.3±8.0), followed by egg white (100.9±6.3) and milk (101.1±7.3).Conclusion: Within the limitations of this study, it appears that aloe vera maintains PDL cell viability better than egg white or milk.

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    APA: Copy

    SHARMA, MEENAKSHI, SHARMA, SANJAY, REDDY, Y.G., MITTAL, RAKESH, AGARWAL, VISHAL, SINGH, CHANCHAL, & SINGH, AMANDEEP. (2015). EVALUATION OF PERIODONTAL LIGAMENT CELL VIABILITY IN THREE DIFFERENT STORAGE MEDIA: AN IN VITRO STUDY. FRONTIERS IN DENTISTRY (JOURNAL OF DENTISTRY OF TEHRAN UNIVERSITY OF MEDICAL SCIENCES), 12(7), 524-531. SID. https://sid.ir/paper/298017/en

    Vancouver: Copy

    SHARMA MEENAKSHI, SHARMA SANJAY, REDDY Y.G., MITTAL RAKESH, AGARWAL VISHAL, SINGH CHANCHAL, SINGH AMANDEEP. EVALUATION OF PERIODONTAL LIGAMENT CELL VIABILITY IN THREE DIFFERENT STORAGE MEDIA: AN IN VITRO STUDY. FRONTIERS IN DENTISTRY (JOURNAL OF DENTISTRY OF TEHRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2015;12(7):524-531. Available from: https://sid.ir/paper/298017/en

    IEEE: Copy

    MEENAKSHI SHARMA, SANJAY SHARMA, Y.G. REDDY, RAKESH MITTAL, VISHAL AGARWAL, CHANCHAL SINGH, and AMANDEEP SINGH, “EVALUATION OF PERIODONTAL LIGAMENT CELL VIABILITY IN THREE DIFFERENT STORAGE MEDIA: AN IN VITRO STUDY,” FRONTIERS IN DENTISTRY (JOURNAL OF DENTISTRY OF TEHRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 12, no. 7, pp. 524–531, 2015, [Online]. Available: https://sid.ir/paper/298017/en

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