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Title

THE EFFECT OF PROGESTERONE ON THE IN VITRO MATURATION OF MOUSE GV OOCYTES

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Abstract

 Introduction: The present study was carried out to investigate the role of different concentrations of PROGESTERONE on the maturation of cumulus oocyte complex (COC) and denuded OOCYTES (DO) during IVM of mouse germinal vesicle (GV) OOCYTES.Materials and Methods: Prepubertal mice (24-28 days old) were primed with 7 IU PMSG. OOCYTES were retrieved from the ovary 48 hours later for IVM. Immature GV OOCYTES were divided into two groups A: DO and B: COC. The OOCYTES were cultured in TCM199 supplemented with antibiotics (50 μg/ml penicillin and 75 mg/ml streptomycin), 0.23 mM pyruvate, 10% FBS, 75 mIU/ml rhFSH, 10 IU/ml hCG and different concentrations of PROGESTERONE (0, 10, 38, 50 or 100 mM). At the end of the culture period, the number of GV OOCYTES, germinal vesicle breakdown (GVBD) and metaphase II (MII) were counted using an inverted microscope.Results: The percentage of MII OOCYTES in COC groups (0, 10, 38, 50 and 100 mM PROGESTERONE) were 85.29, 82.82, 48.43, 14.63 and 0%, respectively. There were significant differences between control and all treatment groups except for the 10 mM concentration (p>0.05). The MII rates in DO groups with different concentrations of PROGESTERONE were 68.80, 66.55, 53.92, 20.79 and 0%, respectively. These rates were statistically significant between control and all treatment groups except for the 10 mM concentration (p>0.05). Also, statistically significant difference was seen among PROGESTERONE treatment groups and the highest inhibition was seen in 100 mM PROGESTERONE in maturation medium (p>0.05). The results showed that maturation rate of COC was significantly higher than that of DO in control group (p<0.05) and PROGESTERONE was more effective to block MII maturation in COC than DO.Conclusion: These data suggested that presence of PROGESTERONE in maturation medium of mouse GV oocyte could not improve maturation rate. However, PROGESTERONE concentrations more than 10 mM in maturation medium resulted in inhibition of GV oocyte maturation in mouse.

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