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RELATIONSHIP BETWEEN REPRODUCTIVE HORMONES AND OXIDATIVE STRESS WITH SPERM DNA INTEGRITY

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Abstract

 Introduction: Sperm DNA integrity is critical for male fertility and its damage contributes to poorer embryo development, etc. REPRODUCTIVE HORMONES are vital to spermatogenesis and maintenance of male reproductive function, and likely play an important role in human sperm DNA integrity, but this relationship is not well understood. Therefore, aim of this study was to examine these relationships.Materials and Methods: In this study, blood and semen samples were collected from men undergoing evaluation for infertility in Avicenna Infertility Clinic (AIC). According to the semen analysis results, the men were divided into two groups: a male factor infertility group (group 1) (n=81) and a non-male factor group (group 2) (n=52). Serum LH, FSH and testosterone were measured by chemiluminescence assay; in addition serum DHEA and estradiol were measured by RIA. SPERM DNA DAMAGE was examined by toluidine blue (TB) staining method. Amount of sperm nuclear protamine was indirectly examined by chromomycin A3 (CM A3) staining method. Total antioxidant capacity (TAC/oxidative stress index) was measurement by spectrophotometer assay. Data were collected and analyzed by SPSS 13 software.Results: FSH, LH, TB and CMA3 were significantly higher in group 1 as compared to group 2. Testosterone, DHEA, estradiol and TAC did not show significant difference between the two groups. In preliminary bivariate analyses TB were positively associated with LH, FSH and CM A3, but inversely associated with count and motility. However, Multivariate linear regression for variables shows sole significant relationship between TB and CM A3.Conclusion: Our results demonstrated that though OXIDATIVE STRESS and REPRODUCTIVE HORMONES may be the causes of SPERM DNA DAMAGE but reduction in the amount of sperm nuclear protamine, as key reason, can lead to SPERM DNA DAMAGE. However, additional studies are needed to investigate whether there are relationships between sperm DNA integrity with REPRODUCTIVE HORMONES and OXIDATIVE STRESS.

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